Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: The differences of optimal vibration frequency for accelerating OTM between the younger group and the older group (A and B) Micro-CT images and statistical analysis showed the orthodontic teeth of the younger rats in L-Vib group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (C) The content of CTX-1 in serum of the younger rats in control group, L-Vib group and H-Vib group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. (D and E) Representative images of TRAP staining of alveolar bone of the younger rats in control group, L-Vib group and H-Vib group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (F) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the younger rats in control group, L-Vib group and H-Vib group. Scale bar: 100 μm. (G) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in L-Vib group were higher than those in control group and H-Vib group. Mean ± SD. n = 3. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (H and I) Micro-CT images and statistical analysis showed the orthodontic teeth of the older rats in H-Vib group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5. ∗ p < 0.05. ∗∗∗ p < 0.001. (J) The content of CTX-1 in serum of the older rats in control group, L-Vib group and H-Vib group was detected by ELISA. Mean ± SD. n = 3–4. ∗ p < 0.05. ∗∗ p < 0.01. (K and L) Representative images of TRAP staining of alveolar bone of the older rats in control group, L-Vib group and H-Vib group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗∗ p < 0.001. (M) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the older rats in control group, L-Vib group and H-Vib group. Scale bar: 100 μm. (N) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in H-Vib group were higher than those in control group and L-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. Statistical significance was determined using the one-way ANOVA.

Article Snippet: The serum levels of CTX-1 and PINP in the rats were quantified using ELISA kits (CUSABIO, CSB-E12776r, CSB-E12774r).

Techniques: Micro-CT, Control, Enzyme-linked Immunosorbent Assay, Staining, Expressing

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: The synergistic effect of vibration and NIR light on OTM (A) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the younger rats in control group, L-Vib group and synergy group. Scale bar: 100 μm. (B) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in synergy group were higher than those in control group and L-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (C and D) Representative images of TRAP staining of alveolar bone of the younger rats in control group, L-Vib group and synergy group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4. ∗ p < 0.05. ∗∗∗ p < 0.001. (E) The content of CTX-1 in serum of the younger rats in control group, L-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. (F and G) Micro-CT images and statistical analysis showed the orthodontic teeth of the younger rats in synergy group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5–6. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (H) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the older rats in control group, H-Vib group and synergy group. Scale bar: 100 μm. (I) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in synergy group were higher than those in control group and H-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (J and K) Representative images of TRAP staining of alveolar bone of the older rats in control group, H-Vib group and synergy group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (L) The content of CTX-1 in serum of the older rats in control group, H-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗∗ p < 0.001. (M and N) Micro-CT images and statistical analysis showed the orthodontic teeth of the older rats in synergy group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5–6. ∗∗∗ p < 0.001. Statistical significance was determined using the one-way ANOVA.

Article Snippet: The serum levels of CTX-1 and PINP in the rats were quantified using ELISA kits (CUSABIO, CSB-E12776r, CSB-E12774r).

Techniques: Control, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Micro-CT

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: Light-vibration coordination accelerates OTM without root resorption and alveolar bone resorption (A and B) Micro-CT images and statistical analysis showed that the moving distance of orthodontic tooth was increased in synergy group of the younger rats after orthodontic force application for 4 weeks. Scale bar: 2 mm. Mean ± SD. n = 5. ∗∗ p < 0.01. (C) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in synergy group were higher than those in control group after orthodontic force application for 4 weeks. Mean ± SD. n = 3. ∗∗∗ p < 0.001. (D and E) Micro-CT images and statistical analysis showed that the moving distance of orthodontic tooth was increased in synergy group of the older rats after orthodontic force application for 4 weeks. Scale bar: 2 mm. Mean ± SD. n = 6. ∗∗ p < 0.01. (F) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in synergy group were higher than those in control group after orthodontic force application for 4 weeks. Mean ± SD. n = 3. ∗∗ p < 0.01. (G) Representative images of HE staining of the longitudinal sections of the maxillary first molar of the younger rats. Scale bar: 500 μm. (H and I) There was no significant difference in the level of root resorption and alveolar bone resorption between the control group and the synergy group of the younger rats. Mean ± SD. n = 4. ns, not significant. (J) The content of PINP in serum of the younger rats in control group, L-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3–4. ∗∗∗ p < 0.001. (K) WB results showed that the protein expression levels of RUNX2 and OSX of the younger rats in synergy group were higher than those in control group. Mean ± SD. n = 3. ∗∗ p < 0.01. (L) Representative images of HE staining of the longitudinal sections of the maxillary first molar of the older rats. Scale bar: 500 μm. (M and N) There was no significant difference in the level of root resorption and alveolar bone resorption between the control group and the synergy group of the older rats. Mean ± SD. n = 3–4. ns, not significant. (O) The content of PINP in serum of the older rats in control group, H-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3–4. ∗∗∗ p < 0.001. (P) WB results showed that the protein expression levels of RUNX2 and OSX of the older rats in synergy group were higher than those in control group. Mean ± SD. n = 3. ∗∗ p < 0.01. Statistical significance was determined using the Student’s t tests one-way ANOVA.

Article Snippet: The serum levels of CTX-1 and PINP in the rats were quantified using ELISA kits (CUSABIO, CSB-E12776r, CSB-E12774r).

Techniques: Micro-CT, Expressing, Control, Staining, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: The differences of optimal vibration frequency for accelerating OTM between the younger group and the older group (A and B) Micro-CT images and statistical analysis showed the orthodontic teeth of the younger rats in L-Vib group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (C) The content of CTX-1 in serum of the younger rats in control group, L-Vib group and H-Vib group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. (D and E) Representative images of TRAP staining of alveolar bone of the younger rats in control group, L-Vib group and H-Vib group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (F) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the younger rats in control group, L-Vib group and H-Vib group. Scale bar: 100 μm. (G) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in L-Vib group were higher than those in control group and H-Vib group. Mean ± SD. n = 3. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (H and I) Micro-CT images and statistical analysis showed the orthodontic teeth of the older rats in H-Vib group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5. ∗ p < 0.05. ∗∗∗ p < 0.001. (J) The content of CTX-1 in serum of the older rats in control group, L-Vib group and H-Vib group was detected by ELISA. Mean ± SD. n = 3–4. ∗ p < 0.05. ∗∗ p < 0.01. (K and L) Representative images of TRAP staining of alveolar bone of the older rats in control group, L-Vib group and H-Vib group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗∗ p < 0.001. (M) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the older rats in control group, L-Vib group and H-Vib group. Scale bar: 100 μm. (N) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in H-Vib group were higher than those in control group and L-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. Statistical significance was determined using the one-way ANOVA.

Article Snippet: PINP ELISA kit , CUSABIO , CSB-E12774r.

Techniques: Micro-CT, Control, Enzyme-linked Immunosorbent Assay, Staining, Expressing

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: The synergistic effect of vibration and NIR light on OTM (A) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the younger rats in control group, L-Vib group and synergy group. Scale bar: 100 μm. (B) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in synergy group were higher than those in control group and L-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (C and D) Representative images of TRAP staining of alveolar bone of the younger rats in control group, L-Vib group and synergy group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4. ∗ p < 0.05. ∗∗∗ p < 0.001. (E) The content of CTX-1 in serum of the younger rats in control group, L-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. (F and G) Micro-CT images and statistical analysis showed the orthodontic teeth of the younger rats in synergy group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5–6. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (H) Immunofluorescent images of COX-2 and IL-6 in alveolar bone of the older rats in control group, H-Vib group and synergy group. Scale bar: 100 μm. (I) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in synergy group were higher than those in control group and H-Vib group. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (J and K) Representative images of TRAP staining of alveolar bone of the older rats in control group, H-Vib group and synergy group, and statistics on the number of osteoclasts. AB, alveolar bone; R, root; PDL, periodontal ligament. Scale bar: 200 μm. Mean ± SD. n = 4–5. ∗ p < 0.05. ∗∗ p < 0.01. ∗∗∗ p < 0.001. (L) The content of CTX-1 in serum of the older rats in control group, H-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3. ∗ p < 0.05. ∗∗∗ p < 0.001. (M and N) Micro-CT images and statistical analysis showed the orthodontic teeth of the older rats in synergy group moved the farthest. Scale bar: 2 mm. Mean ± SD. n = 5–6. ∗∗∗ p < 0.001. Statistical significance was determined using the one-way ANOVA.

Article Snippet: PINP ELISA kit , CUSABIO , CSB-E12774r.

Techniques: Control, Expressing, Staining, Enzyme-linked Immunosorbent Assay, Micro-CT

Journal: iScience

Article Title: Vibration tailored to jawbone density with near-infrared light expedites orthodontic tooth movement

doi: 10.1016/j.isci.2025.114237

Figure Lengend Snippet: Light-vibration coordination accelerates OTM without root resorption and alveolar bone resorption (A and B) Micro-CT images and statistical analysis showed that the moving distance of orthodontic tooth was increased in synergy group of the younger rats after orthodontic force application for 4 weeks. Scale bar: 2 mm. Mean ± SD. n = 5. ∗∗ p < 0.01. (C) WB results showed that the protein expression levels of COX-2 and IL-6 of the younger rats in synergy group were higher than those in control group after orthodontic force application for 4 weeks. Mean ± SD. n = 3. ∗∗∗ p < 0.001. (D and E) Micro-CT images and statistical analysis showed that the moving distance of orthodontic tooth was increased in synergy group of the older rats after orthodontic force application for 4 weeks. Scale bar: 2 mm. Mean ± SD. n = 6. ∗∗ p < 0.01. (F) WB results showed that the protein expression levels of COX-2 and IL-6 of the older rats in synergy group were higher than those in control group after orthodontic force application for 4 weeks. Mean ± SD. n = 3. ∗∗ p < 0.01. (G) Representative images of HE staining of the longitudinal sections of the maxillary first molar of the younger rats. Scale bar: 500 μm. (H and I) There was no significant difference in the level of root resorption and alveolar bone resorption between the control group and the synergy group of the younger rats. Mean ± SD. n = 4. ns, not significant. (J) The content of PINP in serum of the younger rats in control group, L-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3–4. ∗∗∗ p < 0.001. (K) WB results showed that the protein expression levels of RUNX2 and OSX of the younger rats in synergy group were higher than those in control group. Mean ± SD. n = 3. ∗∗ p < 0.01. (L) Representative images of HE staining of the longitudinal sections of the maxillary first molar of the older rats. Scale bar: 500 μm. (M and N) There was no significant difference in the level of root resorption and alveolar bone resorption between the control group and the synergy group of the older rats. Mean ± SD. n = 3–4. ns, not significant. (O) The content of PINP in serum of the older rats in control group, H-Vib group and synergy group was detected by ELISA. Mean ± SD. n = 3–4. ∗∗∗ p < 0.001. (P) WB results showed that the protein expression levels of RUNX2 and OSX of the older rats in synergy group were higher than those in control group. Mean ± SD. n = 3. ∗∗ p < 0.01. Statistical significance was determined using the Student’s t tests one-way ANOVA.

Article Snippet: PINP ELISA kit , CUSABIO , CSB-E12774r.

Techniques: Micro-CT, Expressing, Control, Staining, Enzyme-linked Immunosorbent Assay